Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. The linear detection range of the PicoGreen® assay in the 20/20n extends over three orders of magnitude in DNA concentration - from 450 pg/mLFor DNA quantification with PicoGreen (PG), Quant-iT™ PicoGreen® dsDNA Reagents and Kits (Invitrogen – Molecular Probes, Eugene, OR, USA) were used. 12. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. It must be completely liquified before use by allowing it to come to room temperature. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 86. 50 pg to 2 µg. • Suitable for use in 96- and 384-well plate formats. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Quant-iT™ PicoGreen ® dsDNA Assay Kit. DNA standard curve. 99. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. 3 Q32854 26. Add 5uL of PicoGreen reagent for every 1mL of buffer. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 5 Q32851 15. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Touch devices users use touch & swipe gestures. 文件. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. 2. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. Triplicate 10 μL samples of λ DNA (Ο), E. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Facebook. Bioz Stars score: 86/100, based on 1 PubMed citations. P11495. 2 ng to 100 ng of DNA in the assay tube. quant it picogreen dsdna assay kit reagents. Bioz Stars score: 86/100, based on 1 PubMed citations. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Begin typing to search, use arrow keys to navigate, Enter to select. '. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Rapidly detect as little as 0. 2–400ng/µl (1µl sample in 200µl assay). Prepared DNA concentration of the samples was 250 ng/ mL. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). Supplier: Invitrogen™ P11496. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. 5. Using 20x TE buffer make 1x solution. 22. Skip to main list 美谷分子仪器（上海）有限公司. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. . 9. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Quantify nucleic acids and proteins using four user-selectable fluorescent. Buy from Supplier. Results: DNA concentration values were measured by the “DNA Mamm. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. For duplicate-well droplet. Table 3. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Quantitation Range. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Rapidly detect as little as 0. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 1 mL kit. Rapidly detect as little as 0. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. Bioz Stars score: 86/100, based on 1 PubMed citations. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. 2023-11. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. VIEWS. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 10 x 100 µL. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. 8 Measurement of growth factor content in. 1. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. high-range. Quantitation Range. Picogreen Reagent b. 99. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Rapidly detect as little as 0. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. This assay is designed for use with fluorescence microplate readers. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Quant-iT PicoGreen dsDNA Reagent. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Prepare the assay. 8. • Suitable for use in 96- and 384-well plate formats. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 2–100 ng: 2-1,000 ng: 0. Barcoded amplicons were pooled in. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Quant-iT RiboGreen RNA Reagent The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. 製品概要. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Q33130). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 995 mL TE for the . 5 ng/µL. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. High tolerance for contaminants. No. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. 7 Q33231 6 1,000 41. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Qubit® dsDNA BR Assay Kit. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. For a standard. The libraries were sequenced using the Illumina HiSeq 2500. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). • Suitable for use in 96- and 384-well plate formats. Unit Size. Determination of DNA Yield. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 99. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. Mix PicoGreen and DNA by pippetting up and down 5-10 times. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. Le réactif et les kits de quantification d’ADNdb PicoGreen sont parfaits pour les dosages PCR, les échantillons de biopuces, les dosages de lésions de l’ADN, les dosages d’activité enzymatique, la quantification d’ADN génomique, la mesure de l’ADNdb dans des mélanges complexes et la quantification d’ADN viral. Catalog Number. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. The Molecular Probes PicoGreen®. Louis, MI, USA). dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. BR reagent (Component A) 1. Easy to use UV absorbance alternative assay that can be. 2–100 ng of DNA. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. Rapidly detect as little as 0. 1 mL kit. 0 mL to 200 uL to fit a 96-well microplate format. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. stranded DNA (dsDNA). Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Katalognummer: P11496. Unit Size. • Specific for dsDNA in the presence of RNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 2. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に対応します。. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. For duplicate-well droplet. Figure 8. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. Quant-iT PicoGreen dsDNA Assay Kit. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Mix by gently vortexing for 5 seconds. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 0, 20X c. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Bioz Stars score: 86/100, based on 1 PubMed citations. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. 反应次数. 99 to $1999. Bioz Stars score: 99/100, based on 1 PubMed citations. This kit contains. Quant-iT PicoGreen dsDNA Assay Kit. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. quant it picogreen dsdna assay kit , 2023-10. Product Type. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 3. No. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. Picogreen assays are compatible with most microplate readers and fluorometers. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Picogreen assays are compatible with most microplate readers and fluorometers. Three kits covering a wide range of dsDNA concentrations. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assays are compatible with most microplate readers and fluorometers. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. AccuBlue® RNA Quantitation Kit Details. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Picogreen assay kit (Invitrogen, P7589) a. Pico488 allows to quantitate as little as low pg/mL of dsDNA. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. Materials: 1. 1 mL kit. Reagent Search & Data Services Menu Reagent Search. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. The AccuBlue® High Sensitivity assay is also available without any DNA standards ( 31008 ), for those that wish to use their own standard. Rapidly detect as little as 0. PicoGreen dsDNA quantitation reagents are. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. PicoGreen®試薬アッセイ. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. 99 to $1999. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. • Specific for dsDNA in the presence of RNA. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. 2023-11. 2. 50 pg to 2 µg. Highly selective for dsDNA over ssDNA or RNA. samples as well as standard in the assay itself. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. yields in a method for direct cycle sequencing of PCR products. Price from $9. 2023. For Research Use Only. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 50 pg to 2 µg. 9 Q33230 9 500 28. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Picogreen assays are compatible with most microplate readers and fluorometers. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). 500/525. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. 50 pg to 2 µg. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. • Suitable for use in 96- and 384-well plate formats. Picogreen assays are compatible with most microplate readers and fluorometers. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Price from $9. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Catalog Number. in the assay in order to reduce waste and still achieve comparable results. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. a) 16. Libraries were sequenced on the Illumina NovaSeq. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Measure concentration with a high-sensitivity assay (e. • Suitable for use in 96- and 384-well plate formats. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. Price from $9. Optimized for fluorescence microplate readers. 10. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 2 ng to 100 ng of DNA in the assay tube. 5mL. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. 99 to $1999. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). P7589. 参考資料. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. In addition to the Quant-iT™ dsDNA Broad-Range Assay Kit described here, we also offer the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Cat. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. PicoGreen dsDNA quantitation reagents are. 2. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. 3,201 3. Briefly, 2 µL of sample was diluted in 498 µL TE. 522. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). 5 µL of each extracted sample was used for Picogreen analysis. Unit Size. The reagent must be completely thawed before using it by bringing it to room temperature. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Katalognummer: P11495. Quant-iT dsDNA BR buffer (Component B) 250 ml c. This protocol is a simplified and c. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. Description. no. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. 5) prepared from 20x. 50 pg to 2 µg. Rapidly detect as little as 0. Tweet. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). This kit contains. Figure 2. • Suitable for use in 96- and 384-well plate formats. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay.